基于空间位移拉曼光谱原理，搭建了一套可以实现微小空间位移的装置，确定了最优的空间位移距离为300 μm，用于穿透皮肤表层，减少皮肤表面水份、油脂等污染物的干扰，最终采集到皮肤内层的水份拉曼信号。该装置采用拉曼波数在3 390 cm^-1（水拉曼峰）和2 935 cm^-1（蛋白峰）处的强度之比确定组织中的水-蛋白比例，采用便携式光谱仪使设备便于移动，探头和光谱仪以光纤相连使探头可灵活操作，设计满足临床使用要求。实验中检测了7个人、每人10个共70个不同位置的皮肤光谱，将光谱进行去背景算法处理，然后得到精确的拉曼峰强度。以电学法皮肤水份测试仪测试结果作为对比，以组内相关系数作为一致性分析指标，由单个测量组内相关系数为0.889可以看出，基于空间位移拉曼光谱法的人体活体皮肤含水量检测具有较高可行性。

In this Letter, a miniature wearable Raman spectroscopy system is developed. A wearable fiber-optic probe is employed to help the stable and convenient collection of Raman spectra. A nonlinear partial least squares model based on a multivariate dominant factor is employed to predict the glucose level. The mean coefficients of determination are 0.99, 0.893, and 0.844 for the glucose solution, laboratory rats, and human volunteers. The results demonstrate that a miniature wearable Raman spectroscopy system is feasible to achieve the noninvasive detection of human blood glucose and has important clinical application value in disease diagnosis.